Product Details
| Alternative Name: | CD178, CD95L, APO-1L, TNFSF 6 |
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| Clone: | A11 |
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| Host: | Rat |
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| Isotype: | IgM |
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| Immunogen: | Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178). |
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| UniProt ID: | P41047 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Mouse
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| Applications: | Flow Cytometry, IHC (FS), IHC (PS)
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| Application Notes: | For Western blot, we recommend MAb to FasL (mouse) (H11) (Prod. No. ALX-804-010). |
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| Formulation: | Liquid. Purified antibody in PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Schematic structure of mouse FasL (peptide, aa. 196-220)
Figure: Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody.
Method: Cryostat sections (6-8µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 µg/ml of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H
2O
2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H
20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/ml antibody, wash in PBS, incubate in anti-rat IgM HRP (
ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC)."
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Product Literature References
Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions: I. Viard-Leveugle, et al.; J. Biol. Chem.
278, 16183 (2003),
Abstract;
Full Text
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol.
166, 6964 (2001),
Abstract;
Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy: M. Sata, et al.; Arterioscler. Thromb. Vasc. Biol.
20, 309 (2000),
Abstract;
Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis: Y. Refaeli, et al.; Immunity
8, 615 (1998),
Abstract;
The regulation of CD95 ligand expression and function in CTL: J.H. Li, et al.; J. Immunol.
161, 3943 (1998),
Abstract;
Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?: P. Saas, et al.; J. Clin. Invest.
99, 1173 (1997),
Abstract;
Full Text
Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover: L.E. French, et al.; J. Cell. Biol.
133, 335 (1996),
Abstract;
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science
274, 1363 (1996),
Abstract;
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand: T. Renno, et al.; J. Exp. Med.
183, 431 (1996),
Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol.
7, 1381 (1995),
Abstract;
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